FIGURE 3.

Systemic delivery of adipolin improves glucose metabolism in DIO mice. After 11 weeks of HFD feeding, DIO mice were intravenously treated with Ad-adipolin or the Ad-β-gal control (5.0 × 10⁸ plaque-forming units, total). A, glucose tolerance (upper panel) and insulin tolerance (lower panel) tests were performed in DIO mice treated with Ad-adipolin or the Ad-β-gal control (mean ± S.E., n = 6 in each group). The inset shows the representative blot for plasma adipolin at 3 days after injection of adenoviral vectors (control or adipolin). Adipolin protein levels in plasma (0.2 μl) were determined by Western blot analysis. B, histological analysis of hematoxylin/eosin-stained epididymal white adipose tissue of DIO mice at 16 days after injection of Ad-adipolin or the Ad-β-gal control. Scale bars = 50 μm. Adipocyte cross-sectional areas were quantified using the Image J program (mean ± S.E., n = 6).