FIGURE 4.

Systemic delivery of adipolin attenuates inflammatory responses in adipose tissue of obese mice. A, macrophage accumulation in epididymal fat tissues of DIO mice at 16 days after administration of Ad-adipolin or the Ad-β-gal control. Histological sections were stained with anti-F4/80 antibody. Scale bars = 50 μm. Macrophage infiltration was quantified as the number of F4/80-positive cells/mm² (mean ± S.E., n = 6). B, gene expression of TNFα, IL-1β, and MCP-1 in epididymal adipose tissue from DIO mice treated with Ad-adipolin or the Ad-β-gal control. Transcript levels were measured by quantitative real-time PCR and are expressed relative to 36B4 levels (mean ± S.E., n = 5–6).