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. 2011 Aug 17;286(40):34552–34558. doi: 10.1074/jbc.M111.277319

FIGURE 5.

FIGURE 5.

Adipolin suppresses gene expression of proinflammatory mediators in macrophages. A, effect of adipolin on LPS-stimulated proinflammatory gene expression in macrophages. Mouse peritoneal macrophages were treated with serial dilutions of conditioned medium (CM) from COS-7 cells transduced with Ad-adipolin (CM-A, 1:4, 1:2, and 1:1 dilutions) or the Ad-β-gal control (CM-C; 1:1 dilution) for 18 h and then stimulated with LPS (100 ng/ml) or vehicle for 6 h. The gene expression levels of TNFα, IL-1β, and MCP-1 were determined by quantitative real-time PCR and are expressed relative to 36B4 levels (mean ± S.E., n = 4). *, p < 0.05; **, p < 0.01 versus CM-C. B, adipolin attenuates TNFα-stimulated proinflammatory gene expression in macrophages. Mouse peritoneal macrophages were treated with CM-A (1:1 dilution) or CM-C (1:1 dilution) for 18 h, followed by treatment with TNFα (50 ng/ml) or vehicle for 6 h. Transcript levels were determined by quantitative real-time PCR and are expressed relative to 36B4 levels (mean ± S.E., n = 4).