TABLE 1.
Summary of analytical size exclusion, dynamic light scattering, and SLS measurements
| Mr | Mr ASEC | Mr SLS | Rh (MAD) | % Pd (MAD)a | |
|---|---|---|---|---|---|
| kDa | kDa | kDa | nm | ||
| PDZ-L1-RGSL | 49 | 145 | 5.1 (0.23) | 11.5 (3.30) | |
| N-DH-PH | 48.7 | 4.1 (0.18) | 10.5 (3.50) | ||
| N-DH-PH /RhoA | 69.3 | 4.3 (0.07) | 13.5 (2.35) | ||
| N-DH-PH 4R | 49.3 | 4.4 (0.31) | 13.8 (5.99) | ||
| N-DH-PH 4R/RhoA | 69.9 | 4.4 (0.18) | 12.6 (2.66) | ||
| RGSL-L2-DH-PH | 93.2 | 250 | 6.3 (0.20) | 14.9 (3.84) | |
| RGSL-L2-DH-PH /RhoA | 114.6 | 260 | 6.0 (0.05) | 13.1 (7.55) | |
| RGSL-L2-DH-PH 4R | 92.8 | 7.0 (0.35) | 23.8 (4.95) | ||
| RGSL-L2-DH-PH 4R/RhoA | 115.2 | 6.2 (0.20) | 12.6 (5.05) | ||
| PDZ-L1-RGSL-L2-DH-PH | 118.2 | 420 | 115 | 7.7 (0.22) | 19.9 (2.78) |
| PDZ-L1-RGSL-L2-DH-PH /RhoA | 139.6 | 450 | 137 | 7.2 (0.32) | 18.1 (4.35) |
| PDZ-L1-RGSL-L2-DH-PH 4R | 118.8 | 390 | 7.8 (1.16) | 19.0 (6.05) | |
| PDZ-L1-RGSL-L2-DH-PH 4R/RhoA | 140.2 | 420 | 7.0 (0.45) | 9.74 (1.28) |
a Mr, Mr ASEC, and Mr SLS are molecular weight, molecular weight determined with analytical size exclusion, and molecular weight determined by static light scattering, respectively. Rh and % Pd are the hydrodynamic radius and percent polydispersity, respectively, determined in each case from 5–10 independent measurements. MAD is mean absolute deviation.