FIGURE 4.

Lysosomes are proliferated in ML-II skin fibroblasts. A, LysoTracker and DAPI fluorescent micrographs of normal, ML-II, and enzyme-treated ML-II cells, indicating a reduction in total lysosomal amount. Scale bar, 20 μm. B, to quantify the amount of lysosomes in one cell, the ratio of LysoTracker/DAPI fluorescence was measured with a plate reader after dual staining of a living suspension of normal, ML-II, and enzyme-treated ML-II cells (n = 3). The lysosomal amount was estimated to be accumulated ∼3-fold in ML-II cells. *, p < 0.05, **, p < 0.005.