FIGURE 5.

Intracellular logistics are impaired in ML-II. A, endocytic targeting of ceramide in normal, ML-II, and enzyme-treated ML-II cells was evaluated by BODIPY-ceramide targeting. Micrographs show fluorescence of living cells using confocal microscopy after treatment with BODIPY-ceramide for 30 min. Scale bars, 20 μm. B, micrographs showing results of MP6 receptor antibody uptake testing of normal, ML-II, and enzyme-treated ML-II cells. In normal and enzyme-treated ML-II cells, M6P receptors appear to be cycling and distributing broadly. Arrows indicate the Golgi pattern of M6P receptor localization in ML-II cells. Scale bars, 20 μm.