FIGURE 6.

Enzyme treatment improves autophagic and mitochondrial status. A, LC3 immunoblotting of total lysates of normal, ML-II (non), and enzyme-treated ML-II cells indicates a decrease in the specific autophagosome marker, LC3-II, after enzyme treatment of ML-II cells. B and C, LC3 (B) and MitoTracker (C) staining micrographs of normal, ML-II, and enzyme-treated ML-II cells. Scale bars, 20 μm. Enzyme treatment of ML-II cells decreased the number of LC3-positive large vesicles and showed a remarkable recovery of mitochondrial structure.