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. 2001 Mar 27;98(8):4504–4509. doi: 10.1073/pnas.071631698

Figure 4.

Figure 4

Effect of EGF on number and properties of tunnels. Human keratinocytes were plated on fibrin matrix and cultivated in medium containing hrEGF at different concentrations. After 24 h, cultures were fixed, and the number of helical tunnels was counted and expressed as percent of total cells attached to the fibrin (A). The properties of the tunnels, including the number of turns (B), helical pitch (C), and the length of the track composing the helix (D), were estimated from a total of 30 helical tunnels.