Table 1.
EGF increases helical tunnel formation more strikingly than other forms of keratinocyte motility
| Presence of EGF, 10 ng/ml | Total cells | Surface cells
|
Tunnel-forming cells
|
||
|---|---|---|---|---|---|
| Motile* | Cylindrical | Helical | |||
| Expt. 1, n.c. | − | 235 | 63 (27) | 4 (1.7) | 10 (4.3) |
| + | 398 | 214 (54) | 16 (4.0) | 98 (24.6) | |
| Expt. 2, n.c. | − | 260 | 126 (49) | 4 (1.5) | 13 (5.0) |
| + | 327 | 183 (56) | 11 (3.4) | 87 (27) | |
| Expt. 3, c. | − | 204 | 28 (14) | 0 | 0 |
| + | 187 | 66 (35) | 3 (1.6) | 9 (4.8) | |
All experiments were performed on keratinocyte strain YF29. Inoculated cells: n.c., nonconfluent, rapidly growing; c., confluent, slowly growing. Expt., experiment.
*
Over one cell diameter in 24 h. Numbers in parentheses give percentage of total cells.