FIGURE 3.

Effect of silencing Sab on mitochondrial respiratory control ratio (state III/state IV) and nitrotyrosine formation in APAP-induced hepatotoxicity. A, respiratory control ratio (RCR) at various times after APAP treatment in shlacZ (dashed line (♦))- or shsab (solid line (■))-pretreated mice. Details are provided under “Experimental Procedures.” Error bars represent S.D. *, p = 0.002 versus shsab group (Student's t test; n = 3 or 5 per group). B and C, representative immunohistochemical staining of nitrotyrosine liver tissue from shlacZ (B)- and shsab (C)-pretreated mice after treatment with APAP (n = 3 per group). Scale bars = 100 μm. The inset shows punctate nitrotyrosine staining (arrowhead) after APAP treatment in shlacZ-pretreated mice.