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Effect of nucleotides on the assembly of α-Syn in the presence of Hsc70. A, time courses of α-Syn (100 μm) assembly (○) in the presence of Hsc70 (1 μm) in the absence of nucleotide (▴) and the presence of ATP (0.5 mm, ■) or ADP (0.5 mm, ●) in 50 mm Tris-HCl, pH 7.5, 150 mm KCl, 0.05 mm MgCl2. B, negative stained electron micrographs of α-Syn assemblies at 1000 min, labeled with symbols corresponding to those used for the assembly reactions shown in A. Bar, 0.2 μm. AU, arbitrary units.
