FIGURE 3.

MT1-MMP expression of CD24^neg, CD24^low, and CD24^high cells and effect of proteinase inhibition on three-dimensional ECM invasion. A, left panel, flow cytometric analysis of the MT1-MMP receptor expression on the cell surface of CD24^neg, CD24^low, and CD24^high cells. One representative experiment of three is shown. Right panel, MFI (mean ± S.D., n = 3) of MT1-MMP receptor expression on CD24^neg, CD24^low, and CD24^high cells. B, percentage of invasive cells in the presence of an protease inhibitor mixture (PI), GM6001 inhibitor, or DMSO buffer control and invasion profiles of CD24^neg, CD24^low, and CD24^high cells cultured in the presence of PI (D), GM6001 inhibitor (E), or DMSO buffer control (C) that invaded into three-dimensional ECMs after 3 days of culture. (*, p < 0.05; **, p < 0.05; ***, p < 0.001.)