. 2011 Aug 9;286(40):35218–35226. doi: 10.1074/jbc.M111.275107

TABLE 2.

R_g calculation of α_IIbβ₃ based on EM and SAXS data

	Fraction bent^a	Fraction extended^a	R_g from SAXS experiment	Calculated R_g ensemble^b,c
			nm	nm
Ca²⁺/Mg²⁺	0.91	0.09	5.77	5.60
Mn²⁺/Ca²⁺	0.60	0.40	6.43	6.45
Mn²⁺/Ca²⁺ + L-739758	0.20	0.80	7.30	7.41

^a Taken from the α_IIbβ₃ EM class averages in Fig. 2.

^b R_c² = f_b × R_b² + f_e × R_e² where R_c is calculated R_g ensemble, R_b is average R_g of bent α_IIbβ₃, R_e is average R_g of extended α_IIbβ₃, f_b is fraction bent, and f_e is fraction extended.

^c Applying the above equation in the three experimental conditions (R₁² = 0.91R_b² + 0.09R_e² = 5.77² where R₁ = R_g in Ca²⁺/Mg²⁺, R₂² = 0.60R_b² + 0.40R_e² = 6.43² where R₂ = R_g in Mn²⁺/Ca²⁺, and R₃² = 0.20R_b² + 0.80R_e² = 7.30² where R₃ = R_g in Mn²⁺/Ca²⁺ + L-739758) simplifies to R_b² = 74.81 − 43/57 × R_e² and yields an average calculated R_b of 5.33 ± 0.36 nm and R_e of 7.84 ± 0.31 nm.