FIGURE 5.

Reduced tolcapone induced cell death in neuroblastoma 2A cells transfected with COMT-GFP. A, COMT activities of the 2A cells from six-well plates transfected with COMT-GFP (n = 12) and GFP vector (n = 12) were analyzed and presented in the bar graph. B, COMT activities of the 2A cells from six-well plates transfected with COMT-GFP (n = 12) and GFP vector (n = 12) were treated with 100 μm of tolcapone for 24 h. Tolcapone-induced cell death was analyzed and is presented in the bar graph using the controls treated with the vehicle, dimethyl sulfoxide, as baseline or 0% cell death.