FIGURE 6.

Western blot analysis of M. arborescens Se14. The AAH protein was identified with a specific antibody. Samples were taken at distinct time points, the A₆₀₀ was measured, and the sample was stored for Western blot analysis. The samples were diluted or concentrated to the same A₆₀₀ before analysis. The control culture (C) was cultivated in BHI medium without supplementation. A, influence of ferrioxamine E (75 and 300 nm) on the expression of AAH over the whole growth curve. B, influence of ferrioxamine E (150–600 nm) on the expression of AAH in the exponential growth phase. Samples were taken 15, 30, and 45 min after treatment. C, influence of FeSO₄ (75–300 μm) on the expression of AAH in the exponential growth phase. Samples were taken 15, 30, and 45 min after treatment. D, influence of FeSO₄ (75–300 μm) on the expression of AAH over the whole growth curve. E, influence of Fe²⁺ on the expression of AAH in the exponential growth phase. The culture was treated with FeSO₄ or FeCl₃ (300 μm), and samples were taken after 15 and 30 min. F, influence of iron chelators on the expression of AAH. M. arborescens was cultivated for 20 h in the presence of bathophenanthroline disulfonic acid (25–50 μm) and 2,2′-dipyridyl (150–250 μm).