FIGURE 3.

Dominant negative form of ATF6 suppressed apoptosis induced by active ATF6. A, C2C12 cells grown in six-well plates were transiently co-transfected with GFP or GFP-ATF6(1–360) (0.3 μg) and either the pDsRed vector (control) or DsRed-ATF6(158–360) (dominant negative) (1.8 μg). Apoptosis was assessed by cell morphology. B, CHOP did not induce apoptosis in C2C12 cells. C2C12 cells were transiently transfected with GFP or GFP-tagged CHOP. The apoptotic efficiencies of GFP-ATF6(1–360) and the dominant negative GFP-ATF6(158–360) are included in the bar graph.