. Author manuscript; available in PMC: 2012 Aug 5.

Published in final edited form as: Biochem Biophys Res Commun. 2011 Jul 12;411(3):536–542. doi: 10.1016/j.bbrc.2011.06.166

Table 1.

Primers for site-directed mutagenesis of human glutathione synthetase

Enzyme	DNA sequence^a
D458A	5′-CGAGCATGCAGCTGGTGGTGTGGC-3′
	5′-GCCACACCACCAGCTGCATGCTCG-3′
D458R	5′-CGAGCATGCACGTGGTGGTGTGGC-3′
	5′-GCCACACCACCACGTGCATGCTCG-3′
D458N	5′-CCATCGAGCATGCAAATGGTGGTGTGGC-3′
	5′-GCCACACCACCATTTGCATGCTCGATGG-3′

Underlined, bold bases indicate changed nucleotide positions.