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. 2001 Mar 27;98(8):4510–4515. doi: 10.1073/pnas.081074898

Figure 3.

Figure 3

c-Myc is associated with Sp1 and Sp3. (A) Immunoprecipitation (IP) with Sp1 or Sp3 antibodies was performed with cellular extracts from 10.1-MycER cells before and after addition of 4-HT, followed by immunoblotting (WB) with monoclonal anti-c-Myc antibody (9E10). (B) 293 cells were transiently transfected with mammalian expression constructs encoding T7-tagged c-Myc and Myc Δ7-91 and Myc Δ106–143 and harvested after 48 h. Cell lysates were incubated with agarose-linked T7 antibody for 2 h at 4°C. Bound proteins were subjected to immunoblotting with Sp1 antibody PEP2 (Santa Cruz Biotechnology). TCL, total cell lysate.