Figure 3.

Northern analysis of various marker genes. Total RNA was isolated from G418-resistant cell lines overexpressing DMP1. Northern blot was carried out with 20 μg of RNA. The probes were randomly labeled with [³²P]dCTP. C, Stable MC3T3-E1 and C3H10T1/2 cell lines expressing the “empty vector.” S, Stable MC3T3-E1 and C3H10T1/2 cell lines overexpressing DMP1 in sense orientation. AS, Stable MC3T3-E1 and C3H10T1/2 cell lines overexpressing DMP1 in the antisense orientation. Glyceraldehyde-3-phosphate dehydrogenase was used as the control.