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Suppression of DSP and DMP2 by antisense DMP1. Cell lines overexpressing DMP1 (sense) were infected with Ad5/CMV-DMP1 (antisense) as described in Materials and Methods. Total RNA was isolated (after 24 h), and RT-PCR was performed with gene-specific primers. C, Control cells (with “empty vector”). S, cells overexpressing sense DMP1. S + AS infected with Ad5-CMV-DMP1 (antisense) on cells overexpressing DMP1 (sense). In each case DSP and DMP2 were analyzed by RT-PCR.
