FIG. 8.

LTH fails to increase the medial wall thickness in pulmonary arteries from adult sheep. Representative confocal micrographs from (A) normoxic and (B) LTH sheep arteries. Thickness of (C) anti-alpha-smooth muscle actin layer and (D) total arterial wall (in μm) and their ratio (E) (expressed as a percentage). Bars indicate mean±S.E.M. Images and measurements were made optically using a 63X Apochromat oil immersion objective and confocal laser scanning microscopy approaches in multiple sites in each artery.