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. 2010 Jun;29(3):221–229. doi: 10.1089/hyb.2009.0110

FIG. 1.

FIG. 1.

SDS-PAGE Coomassie blue stained protein gel of antigens used to characterize anti-TDI MAb reactivity. Protein-isocyanate conjugates were separated by SDS-PAGE (5 μg/lane, 6% separation gel). The protein bands were visualized by staining the gel with Coomassie blue (Bio-Rad). Lane 1, Precision Plus Protein standard (Bio-Rad); lane 2, HSA; lane 3, 2,4 TDI-HSA; lane 4, 2,6 TDI-HSA; lane 5, 2,4;2,6 TDI-HSA; lane 6, 2,3 DMPI-HSA; lane 7, 2,5 DMPI-HSA; lane 8, 3,4 DMPI-HSA; lane 9, OTI-HSA; lane 10, PTI-HSA; lane 11, HSA; lane 12, MDI-HSA; lane 13, HDI-HSA; lane 14, 2,4 TITC-HAS; and lane 15, 2,6 TITC-HSA.