Table 2.
Extraintestinal Pathogenic Escherichia coli (ExPEC) Status and Virulence Genotypes Associated with the Nine Two-Locus Sequence Groups (SGs) That Contained Both Meat- and Human-Source E. coli Isolates
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No. of isolates per SGawithin each source and resistance status subgroupb |
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Human |
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Meat |
Stool |
Urine |
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| Phylogenetic group | SG | R | S | R | S | R | S | ExPECc | Extended virulence genotyped |
| A | A-1 | 4 | 7 | 6 | 0 | 0 | 0 | no | n/a |
| A | A-3 | 0 | 1 | 1 | 0 | 0 | 0 | no | n/a |
| A | A-5 | 9 | 5 | 2 | 2 | 0 | 0 | no | n/a |
| A | A-6 | 0 | 1 | 0 | 1 | 0 | 0 | no | n/a |
| A | A-7 | 0 | 1 | 0 | 0 | 0 | 1 | no | n/a |
| B1 | B1-2 | 0 | 1 | 0 | 2 | 0 | 0 | no | n/a |
| B1 | B1-4 | 4 | 4 | 1 | 0 | 0 | 0 | no | n/a |
| B2 | B2-1 | 2 | 0 | 0 | 0 | 0 | 1 | yes | papA, papC, sfa/foc, focG, fim, iutA |
| D | D-1 | 0 | 1 | 0 | 1 | 2 | 1 | yes | papA, papC, fim, kpsM II, iutA |
SGs were defined based on idenical sequence over both loci analyzed. A total of 21 SGs were identified; the nine shown are those that contained both meat and human isolates.
R, resistant to one or more of the three key antimicrobial classes, i.e., MIC > 4/76 mg/L to trimethoprim-sulfamethoxazole; MIC ≥ 32 mg/L to nalidixic acid; and/or MIC ≥ 64 μg/ml to ceftriaxone, MIC ≥ 32 μg/ml to ceftazadime, or MIC ≥ 8 μg/ml to cefpodoxime. S, susceptible to all three antimicrobial classes.
ExPEC, positive for two or more of the following five traits: papA and/or papC (P fimbriae structural subunit and assembly; analyzed jointly), sfa/foc (S and FIC fimbriae), afa/dra (Dr-binding adhesins), iutA (aerobactin system), and kpsM II (group 2 capsule).
Extended virulence genotypes determined only for isolates that tested as ExPEC in the initial screen. n/a, not applicable (non-ExPEC).