Fig. 7.
Immune complex-induced Il-6 and IL-1β secretion. WT and α2-null (KO) FSMCs (5 × 104/well) were stimulated with Listeria, Listeria plus anti-Listeria antibody, or Listeria plus anti-Listeria antibody and serum at 37°C. Cell-free supernatants were collected and analyzed for IL-1β (a) or IL-6 (c) by ELISA. FSMCs were lysed after stimulation as described above, and total cellular content was analyzed by ELISA for IL-1β (b) or IL-6 (d). Results are presented as means ± SEM and experiments were performed in duplicate. p values were determined using Student's t test (** p < 0.01; * p = 0.04).