Fig. 8.

Preformed IL-6 is stored in small vesicles. PNS from WT (a) and α2-null (b) FSMCs (1 × 10⁷) were separated over a 2-layer Percoll gradient (1.05 and 1.12 g/ml). Two hundred-microliter fractions were collected from the top of the gradient. Each fraction was analyzed by ELISA for IL-6 and histamine. c Confocal immunofluorescence analysis detected IL-6 (green; colors refer to online version only) and serotonin (red) within separate and distinct mast cell granules. The image is representative of 3 separate experiments.