Fig. 3.

Inducibility of NT-PGC1α in BAT of R6/2 mice upon metabolic stimulation. Cold challenge produces an increase in the levels of NT-PGC1α and UCP-1 protein in the BAT of WT and R6/2 mice. Representative Western blots (n = 6 animals per group) showing protein expression levels for full-length PGC1α and NTPGC1α (top panel) and α-tubulin (lower panel). Lanes 1 and 2 represent untreated WT mice, lanes 3 and 4 represent untreated R6/2 mice, lanes 5 and 6 represent cold-exposed WT mice, lanes 7 and 8 represent cold-exposed R6/2 mice, lane 9 represents BAT from PGC-1α overexpressor mice, and lanes 10 and 11 contain positive controls for NT-PGC1α and full-length PGC1α.