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. 2011 Oct 4;9(10):e1001169. doi: 10.1371/journal.pbio.1001169

Figure 1. BacA proteins protect S. meliloti in vitro against the antimicrobial activity of NCR247.

Figure 1

(A) NCR247 peptide sequence showing the two S-S bridges between cysteine residues 1 and 2 and 3 and 4. (B) Colony forming ability of the indicated strains was assessed after exposure towards 20 µM of the NCR247 peptide for 3 h. (C) The PI fluorescence of cells was determined by flow cytometry after treatment of cultures with and without the NCR247 peptide for 3 h. Bars represent mean ± SD. The significance value ***p≤0.001 was determined using ANOVA followed by a Student Newman-Keuls post-test and results are representative for at least two independent experiments.