Table 1.
Time | Tissue | Replated 1° HPP-CFC | 2° HPP-CFC | 2° LPP-CFC |
---|---|---|---|---|
E9.5 | Yolk sac | 20 | 3 | 3 |
Embryo proper | 20 | 3 | 7 | |
E10.5 | Yolk sac | 20 | 10 | 9 |
Embryo proper | 20 | 9 | 7 |
Twenty primary HPP-CFC from E9.5 and E10.5 yolk sac and embryo proper were replated individually in double agar dishes at days 6 or 7 of primary culture.