Figure 5. Effect of rhSCL on MLO-Y4 viability.

A) Cells were seeded into chamber slides and treated for 3 days A) untreated, or in the presence of rhSCL at 50 ng/ml (B) or 100 ng/ml (C), or D) in the presence of etoposide (50 µM). Cells were then fixed and their nuclear morphology determined by staining with DAPI and visualization by confocal microscopy. E) Cells were seeded into wells of a 6-well plate and cultured for the times indicated with rhSCL (0–200 ng/ml) or in the presence of etoposide (ETOP) at 50 µM. After the times indicated, cell lysates were prepared and caspase 3 activity determined, as described in Materials and Methods. Data are expressed as means of quadruplicate wells ± standard deviations.