Table II. Masses of the phage TP901–1 baseplate and subcomponents. All the masses reported in this table are expressed in Da. N.D., not determined. Numbers underlined indicate masses determined using the method reported in McKay et al., J. Am. Chem. Soc. 2006, 128, 11433–11442.
TP901–1 | ||||||||
---|---|---|---|---|---|---|---|---|
Tripod | ||||||||
Protein | BppU | BppL | BppL Trimer | BppU Trimer | BppU Trimer + BppL Trimer | BppU Trimer + 2xBppL Trimer | Tripod | |
Observed Mass/Da | 33,972 | 17,934 (17,840) | 53,802 (53,522) | 101,918 | 155,470 | 209,764 | 264,088 | |
Calculated Mass/Da | 33,969 | 17,970a (17,839) | 53,910a (53,517) | 101,907 | 155,817a (155,424) | 209,727a (208,941) | 263,637b (262,458) | |
TP1 | ||||||||
Protein | Dit | BppU | BppL | BppL Trimer | 2× BppL Trimer | 3× BppL Trimer | Tripod | Baseplate |
Observed Mass/Da | 29,053b | 33,843 | 18,216 | 54,646 | 109,290 | 164,001 | 265,995 | 1,769,204 |
Calculated Mass/Da | 29,051 | 33,841 | 18,214 | 54,643 | 109,286 | 163,929 | 265,449 | 1,767,016 |
Dit-BppU | ||||||||
Protein | Dit | BppU | BppU Trimer | Dit x 6 | Dit x 12 | 6xDit + 6xBppU-Trimers | 12xDit + 6xBppU-Trimers | |
Observed Mass/Da | 29,197 | 33,855 | 101,584 | 175,173 | 350,348 | 784,979 | 959,852 | |
Calculated Mass/Da | 29,051 | 33,841 | 101,524 | 175,184 | 350,369 | 784,571 | 959,755 | |
Tal-N (ORF47 residues 1 to 386) | Tal-N monomer | Tal-N dimer | Tal-N trimer | |||||
Observed Mass/Da | 47,425 | 94,852 | 142,623 | |||||
Calculated Mass/Da | 47,523 | 95,046 | 142,569 |
a With (without) ORF48 N-terminal Met.
b Observed in native experiment.