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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Gastroenterology. 2011 Jun 23;141(4):1393–1403.e5. doi: 10.1053/j.gastro.2011.06.040

Figure 4. Insulin alters cholesterol-regulating protein expression in primary hepatocyte cultures.

Figure 4

(A) Levels of sterol-response element binding protein-2 (SREBP-2), (B) low density lipoprotein receptor (LDLR), and (C) bile salt exporter protein (Bsep) expression in primary hepatocytes (whole cell lysates) treated with 0, 0.2, 6.5, and 13.0 ng/ml insulin for 48 h (n=3/grp). As shown in (D), protein expression was normalised to β-actin. (E) Liver receptor homolog-1 (LRH-1) and (F) small heterodimer partner (Shp) mRNA was assessed by RT-PCR. There was insufficient material to prepare nuclear protein extracts. This experiment was conducted three times with analyses in triplicate (n=9/grp total). *P<0.05 between groups.