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miR-622, positive control (U6) and negative control (no probe) ISH analyses were performed in normal colorectal mucosa (A) and a group of CRCs (B). Staining for miR-622 was observed in the epithelium throughout the colonic crypt, but there was no staining in the stromal cells. There was a marked overexpression of mir-622 in the CRC samples compared to the normal colorectal mucosa.
