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. Author manuscript; available in PMC: 2012 Oct 15.
Published in final edited form as: J Immunol. 2011 Sep 14;187(8):4331–4337. doi: 10.4049/jimmunol.1100890

Figure 4.

Figure 4

Lyn interacts with MD-2. (A) HEK293 cells were transiently transfected with MD-2, Flag-TLR4 and CD14 construct. 24h later, cells were left untreated (lanes 1 and 2) or stimulated with LPS (lanes 3–5). Lyn proteins were immunoprecipitated with an anti-Lyn antibody in cell lysates and analyzed by SDS-PAGE and immunoblotted with an anti-Flag Ab (top panel), or an anti-Lyn Ab (lower panel). (B) HEK293 cells were transiently transfected with Myc-TLR4 and Flag-MD-2 and CD14 constructs. 24h later, cells were left untreated (lanes 1 and 2) or stimulated with LPS (lanes 3–4). Lyn was immunoprecipitated with an anti-Lyn Ab in cell lysates, and analyzed by SDS-PAGE and immunoblotted with an anti-Flag Ab. (C) HEK293 cells were transiently transfected with Flag-Lyn and HA-MD-2 construct. 24h later, cells were left untreated (lanes 1 and 2) or stimulated with LPS (lanes 3–5). HA-tagged proteins were immunoprecipitated with an anti-HA Ab in cell lysates and analyzed by SDS-PAGE and immunoblotted with an anti-Flag Ab.