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. Author manuscript; available in PMC: 2012 Oct 15.
Published in final edited form as: J Immunol. 2011 Sep 14;187(8):4331–4337. doi: 10.4049/jimmunol.1100890

Figure 5.

Figure 5

Colocalization of endogenous Lyn with MD-2. HEK293 cells were transiently transfected with plasmids expressing Flag-MD-2. After 24 hrs, cells were immunostained with anti-Lyn (red), anti-Flag (green) antibodies, and nuclear stain DAPI (blue). Some samples were also stained with anti-calnexin (far-red). (A) Colocalization of endogenous Lyn with MD-2. HEK293 cells were transiently transfected with plasmids expressing Flag-MD-2 (yellow-merged image). (B) Lyn and MD-2 colocalize with ER (white-merged image). (C) Lyn and MD-2 colocalize with ER (white-merged image) in the presence of LPS.