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. Author manuscript; available in PMC: 2012 Oct 15.
Published in final edited form as: J Immunol. 2011 Sep 12;187(8):3953–3961. doi: 10.4049/jimmunol.1100524

Figure 2.

Figure 2

SiRNA inhibition of LC3 reduces RSV-induced autophagy induction in BMDC as well as induced cytokine expression. A) Inhibition of autophagosome formation in RSV-infected DC was verified using confocal microscopy of LC3 specific antibody stained DCs (red). B) The percent of DC that were positive for autophagosome formation after RSV infection were assessed using confocal microscopy assisted quantification. The data represents mean ± SE from 4-6 different replicates for each group. C) Expression of innate cytokine mRNAby control and LC3 specific siRNA-treated cellswas assessed at 24hrs post RSV infection by qPCR. Data represents mean ± SEM from 3 replicate treatments and was repeated in a separate experiment with similar results.