Figure 5.

Impaired migration of epithelial cells in Klf5 mutant mice. Tissues from adult control and Klf5^Δ/S mice were examined for alterations in epithelial cell migration. (A) Immunohistochemical staining of the small intestine with lysozyme as a Paneth cell-specific marker. Right panel shows a higher magnification of Klf5^Δ/S staining. The red arrowhead indicates a Paneth cell that has migrated from the crypt base. (B) Immunohistochemical staining with the proliferation marker, Ki67, in the small intestine and colon. Red dotted lines indicate the normal boundaries between proliferative and differentiated compartments. Right panels are higher magnification images of adjacent panels. Green arrowheads indicate Ki67-positive proliferating cells. Red arrowheads indicate Ki67-negative non-cycling cells.