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. Author manuscript; available in PMC: 2012 Oct 15.
Published in final edited form as: Biol Psychiatry. 2011 Jul 14;70(8):744–753. doi: 10.1016/j.biopsych.2011.05.021

Figure 7.

Figure 7

Double label immunohistochemistry for c-Fos and prodynorphin in the NAc shell. (A) Sample photomicrograph from NAc shell showing cytosolic prodynorphin immunoreactivity (brown reaction product), nuclear Fos immunoreactivity (blue open arrow, dark purple reaction product), and Fos + prodynorphin double labeling (green arrows). (B) Fos- and Fos + prodynorphin-positive cells were counted in the NAc shell, and a cell density was determined (# cells/area). Acute salvA increased c-Fos expression, but not in prodynorphin-expressing cells. *p<0.05, **p<0.01 compared to Fos group of corresponding treatment; ##p<0.01 comparing groups under the bars. Bonferroni/Dunn’s correction, N=4–6 rats/group. Abbreviations: Fos, c-Fos; DYN, prodynorphin.