Figure 1. ALDH⁺ and ALDH^bright cells present in human carcinoma cell lines, cell line-derived xenografts and a surgically removed SCCHN lesion.

ALDH⁺ and ALDH^bright were identified by flow cytometry analysis following incubation of cells with ALDEFLUOR® in the presence or absence of DEAB inhibitor. A, Purity of sorted ALDH⁺ and ALDH^bright cells PCI-13 cells for ALDH⁺ cells: Percentage ALDH⁺ cells and ALDH MFI of each re-analzyed sorted population are indicated. The dotted lines in the re-analysis of the sorted populations indicate the gates set for identifying ALDH⁺ cells in the sample using ALDEFUOR + DEAB. B, ALDH⁺ and ALDH^bright cells in the SCCHN PCI-13 cell line, a digest of a surgically removed SCCHN lesion, and MDA-MB-231 breast carcinoma and MIA PaCa-2 pancreatic carcinoma cell lines. The percentages of ALDH⁺ and ALDH^bright cells are listed.