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. Author manuscript; available in PMC: 2012 Oct 15.
Published in final edited form as: J Immunol. 2011 Sep 14;187(8):4170–4177. doi: 10.4049/jimmunol.1101634

Figure 7. Effector and memory T cells are resistant to Treg conversion by the ocular microenvironment.

Figure 7

(A) CD4+ T cells from FoxP3-GFP reporter C57BL/6 mice were given a primary stimulation under Th0, Th17-polarizing, or Th1-polarizing conditions for 4 days (1°). Cells were rested in medium containing IL-2 for 4 days and reactivated with anti-CD3/CD28 in the absence or presence of AH under neutral conditions for 3 days in the presence of IL-2 (2°). Cells pulsed with PMA/ionomycin were analyzed for intracellular cytokines. Note: cells given 1° stimulation under non-polarizing conditions produced little or no IL-17, therefore, only IFN-γ staining is shown. (B) Memory or naïve CD4+FoxP3- T cells were purified from naïve FoxP3-GFP reporter C57BL/6 mice on the basis of CD44 expression. Cells were stimulated with anti-CD3/CD28 in the absence or presence of AH for 3 days. The level of FoxP3 expression was determined by flow cytometry. Data are representative of 5 experiments (A) and 3 experiments (B).