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. Author manuscript; available in PMC: 2012 Oct 15.
Published in final edited form as: J Immunol. 2011 Sep 14;187(8):4219–4228. doi: 10.4049/jimmunol.1100826

Figure 6. IFN-γ-deficient naïve and Ad5E1 clone 2.1 tumor-bearing eyes do not express IL-17, IL-6, CD8, or perforin.

Figure 6

Non-tumor-bearing and Ad5E1 clone 2.1 tumor-bearing eyes of C57BL/6, IFN-γ KO and SCID mice were harvested. RNA was isolated and converted to cDNA. mRNA expression of IL-17 (A), IL-6 (B), CD8 (C) and perforin (D) was determined by qPCR. Samples were normalized to GAPDH and compared to IL-6 KO eye. As positive controls for IL-17 and IL-6, Th17-polarized CD4+ T cells and RAW 264.6 cells polarized into a Th17 or M1 phenotype, respectively. Data are a representative of two independent experiments. TB, tumor-bearing; n.d., not detected.