Figure 5.

AMP kinase partially mediates cell death induced by metformin in melanoma. A375 cells were transfected with combination of α1 and α2 AMPK siRNAs or with control siRNA. The cells were next treated for 96 h with indicated concentration of metformin. (a) AMPKα subunit expression, LC3b expression and PARP cleavage were analyzed by western blotting. HSP60 was used as a loading control. Quantification of western blots were performed using Fuji Film, Multi Gauge. (b) FACS analysis using propidium iodide was used to determine cell death. The results are expressed as percentage of cell death compared with control. Data are expressed as percentage of apoptotic cells and represent the mean±S.D. of three independent experiments. Significantly different from the Si-control transfected cells ^#P<0.05