Fig. 4.

In vitro attachment of different G. lamblia lines. Different G. lamblia isolates and lines were added to fresh culture tubes and incubated for the indicated times (A) or for 2 h (B) or were coincubated for 0.5 and 2 h with confluent monolayers of Caco-2 cells (C). Numbers of attached and nonattached trophozoites were determined separately and were used to calculate total numbers in the cultures. Data are means ± SEM of the percentage of attached relative to total trophozoites in each group from 3 to 5 separate experiments. *, P < 0.05 (t test) relative to each parental line; #, P < 0.05 (t test) relative to WB-M1 in panel C.