Table 2.
Oligonucleotides used in this study
| Name | Sequence (5′–3′) |
|---|---|
| RTA2S1 | TGACTTTTTTCTAGCCAACCTAGAGTATTAGATTTGTTTCTCCATCATCAACAACTAAGAAACTTTTCTTGGTGTACCAGTTCATTCCCACCCTTCAACTGAAGCTTCGTACGCTGCAGGTC |
| RTA2S2 | GACAAATATCGAAATTGGTCCTAATATTAACTCTATCAGAATAAAAACTGTTTATTGCATTTACTATCTATCTAGAACAATACTTAATCTAATGCATAACTCTGATATCATCGATGAATTCGAG |
| V5-RTA2 | TCAGGATCACCACAAACACAG |
| V3-RTA2 | TGGTTGCTTTTGCTGGATCT |
| RTA2overF | GGGACAAGTTTGTACAAAAAAGCAGGCTTGATGAGTGAAATCTTGAATTATTTATCTTCG |
| RTA2overR | GGGACCACTTTGTACAAGAAAGCTGGGTTAACTTACTATTTAAACAATGTATGTCATT |
| CEF3_F | GATCACAATTGGGTCCAAGG |
| CEF3_R | AGCAGCGGCAATCTTGTTAC |
| FRE7_F | TCGTTACGCCGATACTTGTG |
| FRE7_R | AATCTCCGTTTGCACCCTTC |
| MET15_F | GGAGATTGCCTCAAACTTGG |
| MET15_R | TGGTAACACCAGAAGCCAAC |
| LYS4_F | CGAAAACCAAACCAGGTGAC |
| LYS4_R | TGTGGCTGCTTGTTCTCTTG |
| GNP1_F | CGACTGATGCATTGGGTTAC |
| GNP1_R | AGCATCTGGTTTGGAAGCAC |
| GAP1_F | ATGGCACCTAAATGGACAGG |
| GAP1_R | TGAAAGCAACCACCTGTTTC |
| CAN1_F | GCCTTTACTGCTGCATTTGG |
| CAN1_R | TGAGATTAACCCTGCGGTTG |
| RTA2_F | GGGGTGGATATTTGTTCACG |
| RTA2_R | ACCCAAACTGGATGGAATGG |
| PTR2_F | GGGTTCATGTTTGGATCTGG |
| PTR2_R | AACATGGACCGGCTTTGTAG |
| orf19.1799_F | TATCTGTGGGGTCATCATCG |
| orf19.1799_R | AGCACGATCAACGTATCCAC |