Fig. 1.

Validation of AS HTS assay. Antifungal activity of different sources of amphotericin B in the HeLa cell-based activity-selectivity assay. A 2-fold serial dilution of amphotericin B (10 different concentrations) was used to validate the assay. The four different lines represent four different batches of amphotericin B from different suppliers (Tü, Boehringer/Roche catalog no. 1081497 [no longer available]; A4888 and A2411, Sigma-Aldrich, Taufkirchen, Germany; BRL18, Gibco/Invitrogen catalog no. 15290-018). The IC₅₀ represents the concentration of the compound required for half-maximal metabolization of fluorescein diacetate after 5 days incubation in the presence of C. albicans. One hundred percent metabolization is determined by using controls without C. albicans and without amphotericin B.