Table 4.
Differentially expressed genes of EMC120B12-treated C. albicans SC5314a
| Gene group and gene | Function | Fold expression | Adjusted P value |
|---|---|---|---|
| Role in ergosterol biosynthesis | |||
| ERG1 | Squalene epoxidase, catalyzes epoxidation of squalene to 2,3(S)-oxidosqualene in the ergosterol biosynthetic pathway | 4.34 | 0.000108 |
| ERG10 | Protein similar to acetyl-CoA acetyltransferase, role in ergosterol biosynthesis | 2.60 | 0.000948 |
| ERG11 | Lanosterol 14-alpha-demethylase, member of cytochrome P450 family that functions in ergosterol biosynthesis | 8.41 | 0.000000 |
| ERG13 | Protein similar to S. cerevisiae Erg13p, which is involved in ergosterol biosynthesis | 4.91 | 0.000000 |
| ERG2 | C-8 sterol isomerase, enzyme of ergosterol biosynthesis pathway | 2.96 | 0.006752 |
| ERG24 | C-14 sterol reductase, has a role in ergosterol biosynthesis | 3.32 | 0.001618 |
| ERG25 | Putative C-4 methyl sterol oxidase with role in C-4 demethylation of ergosterol biosynthesis intermediates, based on similarity to Saccharomyces cerevisiae Erg25p | 2.64 | 0.000193 |
| ERG251 | Predicted ORF in assemblies 19, 20, and 21; ERG251 appears to be an allele or second copy of the ERG25 gene (39) | 4.66 | 0.000024 |
| ERG26 | C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis | 2.09 | 0.000224 |
| ERG3 | C-5 sterol desaturase | 6.95 | 0.000036 |
| ERG4 | Protein described to be similar to sterol C-24 reductase | 3.08 | 0.001412 |
| ERG5 | Putative C-22 sterol desaturase, fungal C-22 sterol desaturases are cytochrome P450 enzymes of ergosterol biosynthesis | 3.05 | 0.000069 |
| ERG6 | Delta(24)-sterol C-methyltransferase, converts zymosterol to fecosterol in ergosterol biosynthesis by methylating position C-24 | 11.36 | 0.000028 |
| ERG9 | Putative farnesyl-diphosphate farnesyl transferase (squalene synthase) involved in the sterol biosynthesis pathway | 2.57 | 0.022424 |
| UPC2 | Transcription factor involved in regulation of ergosterol biosynthetic genes and sterol uptake | 3.47 | 0.000159 |
| Described to show changed transcript levels in response to antimycotics | |||
| CHT2 | Chitinase | −3.90 | 0.000036 |
| CRH11 | Putative ortholog of S. cerevisiae Crh1p | 2.02 | 0.006851 |
| CSH1 | Member of aldo- and ketoreductase family, similar to aryl alcohol dehydrogenases | 2.12 | 0.005475 |
| DDR48 | Immunogenic stress-associated protein | 13.84 | 0.000000 |
| FRP1 | Predicted ferric reductase | 2.01 | 0.000820 |
| FTH1 | Protein not essential for viability, similar to S. cerevisiae Fth1p (putative high-affinity iron transporter for intravacuolar stores of iron) | 2.03 | 0.000472 |
| HYR1 | Nonessential, GPI anchored, predicted cell wall protein | 2.72 | 0.003792 |
| NHP6A | Protein described to be a nonhistone chromatin component | −2.04 | 0.001358 |
| orf19.6688 | Decreased transcription is observed upon benomyl treatment or in an azole-resistant strain that overexpresses MDR1 | −3.39 | 0.000147 |
| orf19.7504 | Predicted ORF in assemblies 19, 20, and 21 | 2.46 | 0.007689 |
| orf19.3475 | Described to be a Gag-related protein | −2.20 | 0.046451 |
| orf19.3737 | Predicted ORF in assemblies 19, 20, and 21 | 2.15 | 0.025789 |
| PGA7 | Protein described to be a putative precursor of a hyphal surface antigen | 2.46 | 0.000472 |
| PHR2 | Glycosidase, role in cell wall structure | 4.38 | 0.000116 |
| POL30 | Protein described as proliferating cell nuclear antigen (PCNA) | −2.00 | 0.006851 |
| RTA2 | Putative floppase | 4.18 | 0.000312 |
| RTA3 | Similar to S. cerevisiae Rta1p (role in 7-aminocholesterol resistance) and Rsb1p (flippase) | 3.18 | 0.030754 |
| Other functions | |||
| ARO10 | Aromatic decarboxylase of the Ehrlich fusel oil pathway of aromatic alcohol biosynthesis | −5.25 | 0.000734 |
| CDG1 | Protein described to be similar to cysteine dioxygenases | −2.04 | 0.000281 |
| CHA1 | Protein similar to serine/threonine dehydratases, catabolic | −2.46 | 0.000734 |
| CIP1 | Protein with slight similarity to plant isoflavone reductase | −2.19 | 0.026148 |
| FAV1 | Protein with weak similarity to S. cerevisiae Fus2p | 2.04 | 0.028653 |
| GCV2 | Putative protein of glycine catabolism | −2.18 | 0.010078 |
| HSP31 | Protein repressed during the mating process | −2.83 | 0.016218 |
| HTA2 | Protein described as histone H2A | −2.32 | 0.003792 |
| MAL2 | Alpha-glucosidase that hydrolyzes sucrose | −2.96 | 0.000166 |
| PGA23 | Putative GPI-anchored protein of unknown function | 5.50 | 0.000009 |
| PGA26 | Putative GPI-anchored protein of unknown function | −3.35 | 0.000260 |
| PST3 | Putative flavodoxin | 2.10 | 0.000028 |
| RBT1 | Putative cell wall protein with similarity to Hwp1p | −2.63 | 0.000177 |
| SET3 | Protein similar to S. cerevisiae Set3p, which is an NAD-dependent histone deacetylase | 3.19 | 0.000005 |
| Predicted ORF, protein of unknown function | |||
| MAL31 | Putative protein of unknown function | −2.75 | 0.003792 |
| orf19.2125 | Predicted ORF in assemblies 19, 20, and 21 | 3.12 | 0.000159 |
| orf19.2989 | Predicted ORF in assemblies 19, 20, and 21 | −2.34 | 0.020304 |
| orf19.4476 | Predicted ORF in assemblies 19, 20, and 21 | 2.34 | 0.007689 |
| orf19.5799 | Predicted ORF in assemblies 19, 20, and 21 | 2.59 | 0.000004 |
| orf19.6840 | Predicted ORF in assemblies 19, 20, and 21 | 2.43 | 0.000285 |
| orf19.6852.1 | ORF added to assembly 21 on the basis of comparative genome analysis | 2.09 | 0.019724 |
| orf19.7455 | Predicted ORF in assemblies 19, 20, and 21 | 3.07 | 0.000159 |
| orf19.90 | Predicted ORF in assemblies 19, 20, and 21 | 2.93 | 0.000633 |
a
Differential expression was determined by comparison to untreated reference samples. Statistical significance was an adjusted P value of ≤0.05; positive values, mRNA level higher than the reference state; negative values, mRNA level lower than the reference state. acetyl-CoA, acetyl coenzyme A; GPI, glycosylphosphatidylinositol.