Table 1.
Antimicrobial susceptibility determination for P. aeruginosa clinical isolate, wild-type P. aeruginosa PAO1, PAO1 harboring natural plasmid p41437, PAO1 harboring recombinant plasmid pOXA-198, E. coli reference strain TOP10, and TOP10 harboring pOXA-198 or p41437
| Antibiotica | MICb (μg/ml) |
||||||
|---|---|---|---|---|---|---|---|
|
P. aeruginosa |
E. coli |
||||||
| PA41437 | PAO1 | PAO1 p41437 | PAO1 pOXA-198 | TOP10 | TOP10 p41437 | TOP10 pOXA-198 | |
| TIM | >256 | 24 | >256 | >256 | 2 | >256 | >256 |
| PIP | 24 | 6 | 32 | 24 | 1.5 | 4 | 32 |
| TZP | 16 | 3 | 24 | 24 | 1 | 4 | 16 |
| FEP | 24 | 3 | 12 | 12 | 0.064 | 0.064 | 0.125 |
| CAZ | 1.5 | 2 | 2 | 2 | 0.125 | 0.125 | 0.125 |
| IPM | >32 | 1.5 | 3 | 3 | 0.38 | 0.5 | 0.5 |
| MEM | 12 | 0.38 | 1.5 | 1.5 | 0.032 | 0.047 | 0.032 |
| ATM | 4 | 4 | 4 | 4 | 0.032 | 0.047 | 0.047 |
| GEN | 12 | 1.5 | 6 | >256c | 0.25 | 0.5 | 32c |
| TOB | 96 | 0.5 | 32 | 0.75 | 0.38 | 6 | 0.75 |
| AMK | 96 | 3 | 64 | 3 | 0.75 | 12 | 0.75 |
| CIP | >32 | 0.094 | 0.094 | 0.094 | <0.002 | <0.002 | <0.002 |
| CST | 1.5 | 1.5 | 2 | 2 | 0.5 | 0.5 | 0.5 |
a
TIM, ticarcillin-clavulanic acid; PIP, piperacillin; TZP, piperacillin-tazobactam; FEP, cefepime; CAZ, ceftazidime; IPM, imipenem; MEM, meropenem; ATM, aztreonam; GEN, gentamicin; TOB, tobramycin; AMK, amikacin; CIP, ciprofloxacin; CST, colistin.
b
MICs were determined by Etest. Values in boldface correspond to significant changes of MICs.
c
High gentamicin MICs are due to the selecting marker (AAC(3)-I) of the pUCP24 shuttle vector.