Fig. 1.
DPP derivatives do not interfere with cell-free PrPres formation and do not dissolve existing PrPSc aggregates. (A) Controls were performed as follows: PrPC in a 1:10 dilution without PrPSc or PK (lane 1), PrPC without PrPSc incubated with PK (lane 2), and PrPC incubated with PrPSc but stopped immediately by freezing (t0 control, lane 3). Lane 4 shows the PrPres fragments after incubation for 72 h with scrapie strain Me7 in the presence of 100 μM DPP-1 (lane 5), 100 μM DPP-2 (lane 6), or 100 μM DPP-3 (lane 7). Detection of PrPC and PrPres fragments was carried out with MAb 3F4. (B) The membrane in panel A was stripped and reincubated with PAb Ra10 for the detection of PrPSc seeds in lanes 3 to 7.