Table 1.

INI off-rate from IN-DNA complexes at 37°C

IN	koffa (s−1) (×10−6)			Relative koff vs wild type
	DTG	RAL	ELV	DTG	RAL	ELV
Wild type	2.7 ± 0.4	22 ± 2	71 ± 4	1.0	1.0	1.0
E92Q	11.4 ± 0.3	59 ± 9	430 ± 20	4.2	2.7	6.1
E138K	2.3 ± 0.2	17 ± 0.3	52 ± 1	0.9	0.8	0.7
G140S	9.6 ± 0.8	44 ± 3	180 ± 20	3.6	2.0	2.5
Y143C	3.2 ± 0.1	96 ± 4	91 ± 2	1.2	4.4	1.3
Y143H	4.4 ± 0.2	78 ± 2	120 ± 6	1.6	3.5	1.7
Y143R	4.6 ± 0.3	176 ± 4	116 ± 5	1.7	8.0	1.6
Q148H	37 ± 3	1,160 ± 120	1,130 ± 140	13.7	53	16
Q148K	18 ± 5	730 ± 130	NDb	6.7	33
Q148R	21 ± 2	480 ± 80	ND	7.8	22
N155H	20 ± 2	300 ± 80	500 ± 140	7.4	14	7.0
E92Q/N155H	49 ± 3	770 ± 70	ND	18	35
E138K/Q148R	53 ± 10	900 ± 340	ND	20	41
G140S/Q148H	58 ± 8	1,130 ± 210	ND	22	51

^ak_off values represent means and standard deviations for 3 to 8 independent experiments.

^bND, not determined due to low signal with [³H]ELV.