Table 4.
Cytoplasmic peptidoglycan precursors synthesized by E. faecium BM4158 (vanG) and E. faecium UCN71 (vanN)
| Strain | Culturea | Amount (%) of indicated peptidoglycan precursorb |
||
|---|---|---|---|---|
| UDP-MurNAc-tetrapeptide | UDP-MurNAc-pentapeptide (d-Ala) | UDP-MurNAc-pentapeptide (d-Ser) | ||
| BM4518 | Uninduced | <1 | 97 ± 1 | 3 ± 1 |
| Vancomycin | 1 ± 3 | 53 ± 3 | 46 ± 4 | |
| UCN71 | Uninduced | 47 ± 3 | 29 ± 1 | 28 ± 1 |
| Vancomycin | 44 ± 4 | 24 ± 1 | 32 ± 2 | |
a
Four micrograms per milliliter of vancomycin was used.
b
Peptidoglycan synthesis was inhibited by the addition of ramoplanin to the cultures for 15 min (5).