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Determination of the optimal concentration of CHAPS for solubilizing r-RVG from Sf-9 cells. The r-RVG-expressing Sf-9 cells were treated with buffer 1, containing various concentrations of CHAPS (from 0.05 to 3%), for 30 min at RT (23 to 25°C). The G content in the supernatant was quantified by IC-ELISA and the results, expressed in μg, are the means ± SDs of results from three different experiments.
